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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may lead to tumorigenesis. This protein was found to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two added splice variants had been claimed but thorough nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind especially on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This manual outlines additional feasible optimization of mobile and immunoassay parameters to be certain the absolute best outcomes are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the very best results are...
Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs for the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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